دراسة تأثير قطبية المذيب على قيمة عامل الحماية من الشمس لمستخلصات إكليل الجبل المُحضرة بمذيبات مختلفة

شعيب الأحمد; نوار بدور; شهد ملاح; سيدرا الحسن

doi:10.5281/zenodo.21787508

Authors

Dr. Shoeb Alahamad Al Wataniya Private University
Dr. Nawar Bdoor Al Wataniya Private University
Shahd Almalah Al Wataniya Private University
Sedra Alhasan Al Wataniya Private University

DOI:

https://doi.org/10.5281/zenodo.21787508

Keywords:

Rosmarinus Officinalis, Sun Protection Factor, Ultrasonic, Extraction Yield, Solvent Polarity

Abstract

This research aims to study the effect of different extraction solvents (water, ethanol 70%, and hexane) on the value of sun protection factor (SPF) of rosemary (Rosmarinus officinalis) extracts obtained by ultrasonic extraction technique. Water produced the highest extract yield at 17.88%, followed by 13.46% for ethanol 70%, and 2.04% for hexane.

Among the three extracts, ethanol 70% produced the strongest photoprotective effect, achieving an SPF of 35.18. This high SPF is attributed to its rich content of phenolic and flavonoids compounds, known for their strong UV-absorbing properties and antioxidant activity. The water extract demonstrated moderate protection with an SPF of 17.62, reflecting its abundance of polar compounds such as phenols and tannins. In contrast, the hexane extract exhibited minimal protection (SPF 7.52) due to its low yield and limited presence of UV-absorbing molecules.

Overall, the findings show that solvent polarity strongly influences the SPF value of rosemary extracts, with ethanol 70% emerging as the most effective solvent.

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References

[1] M. G. Rahbardar and H. Hosseinzadeh, “Therapeutic effects of rosemary (Rosmarinus officinalis L.) and its active constituents on nervous system disorders,” Iranian Journal of Basic Medical Sciences, vol. 23, no. 9, p. 1100, Sep. 2020, doi: 10.22038/IJBMS.2020.45269.10541.

[2] G. Nieto, G. Ros, and J. Castillo, “Antioxidant and antimicrobial properties of rosemary (Rosmarinus officinalis L.): A review,” Medicines, vol. 5, no. 3, Art. no. 98, Sep. 2018, doi: 10.3390/medicines5030098.

[3] J. P. Veenstra and J. J. Johnson, “Rosemary (Salvia rosmarinus): Health-promoting benefits and food preservative properties,” International Journal of Nutrition, vol. 6, no. 4, p. 1, Jun. 2021, doi: 10.14302/issn.2379-7835.ijn-21-3874.

[4] M. Michalak, “Plant-derived antioxidants: Significance in skin health and the ageing process,” International Journal of Molecular Sciences, vol. 23, no. 2, Art. no. 585, Jan. 2022, doi: 10.3390/ijms23020585.

[5] M. Michalak, “Plant extracts as skin care and therapeutic agents,” International Journal of Molecular Sciences, vol. 24, no. 20, Art. no. 15444, Oct. 2023, doi: 10.3390/ijms242015444.

[6] S. Alahmad, N. bdoor, Z. anka, S. chami, and M. naser aldeen, “Study on the extraction of the local Portulaca oleracea plant and evaluation of the sun protection factor (SPF)of both aqueous and ethanolic extracts,” Journal of Al-Wataniya Private University, vol. 3, no. 1, pp. 83–95, Jun. 2025, doi: 10.5281/zenodo.20267608.

[7] A. Verma et al., “Skin protection from solar ultraviolet radiation using natural compounds: A review,” Environmental Chemistry Letters, vol. 22, no. 1, pp. 273–295, Sep. 2023, doi: 10.1007/s10311-023-01649-4.

[8] R. S. Hussein, S. Bin Dayel, O. Abahussein, and A. A. El-Sherbiny, “Influences on skin and intrinsic aging: Biological, environmental, and therapeutic insights,” Journal of Cosmetic Dermatology, vol. 24, no. 2, Art. no. e16688, Feb. 2024, doi: 10.1111/jocd.16688.

[9] T. Breakell et al., “Ultraviolet filters: Dissecting current facts and myths,” Journal of Clinical Medicine, vol. 13, no. 10, Art. no. 2986, May 2024, doi: 10.3390/jcm13102986.

[10] P. G. Carvalho, R. Isla Naveira, L. I. Granone, C. B. Mendive, A. E. Massa, and M. S. Churio, “A comparative review of natural and synthetic UV filters: Gadusol and benzophenone-3 as representative examples,” Environmental Advances, vol. 13, Art. no. 100404, Oct. 2023, doi: 10.1016/j.envadv.2023.100404.

[11] F. Li Pomi et al., “Rosmarinus officinalis and skin: Antioxidant activity and possible therapeutical role in cutaneous diseases,” Antioxidants, vol. 12, no. 3, Art. no. 680, Mar. 2023, doi: 10.3390/antiox12030680.

[12] M. Loussouarn, A. Krieger-Liszkay, L. Svilar, A. Bily, S. Birtić, and M. Havaux, “Carnosic acid and carnosol, two major antioxidants of rosemary, act through different mechanisms,” Plant Physiology, vol. 175, no. 3, pp. 1381–1394, Nov. 2017, doi: 10.1104/pp.17.01183.

[13] P. M. D. J. Fernando et al., “Rosmarinic acid attenuates cell damage against UVB radiation-induced oxidative stress via enhancing antioxidant effects in human HaCaT cells,” Biomolecules & Therapeutics, vol. 24, no. 1, pp. 75–84, 2016, doi: 10.4062/biomolther.2015.069.

[14] A. Pérez-Sánchez et al., “Protective effects of citrus and rosemary extracts on UV-induced damage in skin cell model and human volunteers,” Journal of Photochemistry and Photobiology B: Biology, vol. 136, pp. 12–18, Jul. 2014, doi: 10.1016/j.jphotobiol.2014.04.007.

[15] N. Sánchez-Marzo, A. Pérez-Sánchez, E. Barrajón-Catalán, J. Castillo, M. Herranz-López, and V. Micol, “Rosemary diterpenes and flavanone aglycones provide improved genoprotection against UV-induced DNA damage in a human skin cell model,” Antioxidants, vol. 9, no. 3, Art. no. 255, Mar. 2020, doi: 10.3390/antiox9030255.

[16] A. Nn, “A review on the extraction methods used in medicinal plants: Principles, strengths, and limitations,” Medicinal & Aromatic Plants, vol. 4, no. 3, pp. 3–8, 2015, doi: 10.4172/2167-0412.1000196.

[17] O. Yesil-Celiktas, G. Girgin, H. Orhan, H. J. Wichers, E. Bedir, and F. Vardar-Sukan, “Screening of free radical scavenging capacity and antioxidant activities of Rosmarinus officinalis extracts with focus on location and harvesting times,” European Food Research and Technology, vol. 224, no. 4, pp. 443–451, Feb. 2007, doi: 10.1007/s00217-006-0306-0.

[18] N. Dhouibi et al., “Impact of the extraction method on the chemical composition and antioxidant potency of Rosmarinus officinalis L. extracts,” Metabolites, vol. 13, no. 2, Art. no. 290, Feb. 2023, doi: 10.3390/metabo13020290.

[19] P. C. Sadek, The HPLC Solvent Guide. New York, NY, USA: Wiley, 1996.

[20] R. Kaur, T. B. Gupta, J. Bronlund, and L. Kaur, “The potential of rosemary as a functional ingredient for meat products: A review,” Food Reviews International, vol. 39, no. 4, pp. 2212–2232, 2023, doi: 10.1080/87559129.2021.1950173.

[21] F. Chemat, N. Rombaut, A. G. Sicaire, A. Meullemiestre, A. S. Fabiano-Tixier, and M. Abert-Vian, “Ultrasound-assisted extraction of food and natural products: Mechanisms, techniques, combinations, protocols, and applications—A review,” Ultrasonics Sonochemistry, vol. 34, pp. 540–560, Jan. 2017, doi: 10.1016/j.ultsonch.2016.06.035.

[22] J. Hayat et al., “Phytochemical screening, polyphenols, flavonoids and tannin content, antioxidant activities, and FTIR characterization of Marrubium vulgare L. from two different localities of northeast Morocco,” Heliyon, vol. 6, no. 11, Art. no. e05609, Nov. 2020, doi: 10.1016/j.heliyon.2020.e05609.

[23] G. L. P. Ramya, P. M. Vasanth, P. V. Prasad, and V. Sarath Babu, “Qualitative phytochemical screening tests of Alpinia,” World Journal of Pharmaceutical Research, vol. 8, no. 5, p. 1064, 2019, doi: 10.20959/wjpr20195-14624.

[24] K. Godlewska et al., “Effect of botanical extracts on the growth and nutritional quality of field-grown white head cabbage (Brassica oleracea var. capitata),” Molecules, vol. 26, no. 7, Art. no. 1992, 2021, doi: 10.3390/molecules26071992.

[25] C. Malsawmtluangi, D. K. Nath, I. Jamatia, E. Z. Lianhimgthangi, and L. Pachuau, “Determination of sun protection factor (SPF) number of some aqueous herbal extracts,” Journal of Applied Pharmaceutical Science, vol. 3, no. 9, pp. 150–151, Sep. 2013, doi: 10.7324/JAPS.2013.3925.

[26] R. M. Sayre, P. P. Agin, G. J. LeVee, and E. Marlowe, “A comparison of in vivo and in vitro testing of sunscreening formulas,” Photochemistry and Photobiology, vol. 29, no. 3, pp. 559–566, 1979, doi: 10.1111/j.1751-1097.1979.tb07090.x.

[27] S. D. Sarker and L. Nahar, Chemistry for Pharmacy Students: General, Organic and Natural Product Chemistry. Chichester, U.K.: Wiley, 2007, p. 383.

[28] A. Plaskova and J. Mlcek, “New insights into the application of water or ethanol-water plant extracts rich in active compounds in food,” Frontiers in Nutrition, vol. 10, Art. no. 1118761, Mar. 2023, doi: 10.3389/fnut.2023.1118761.

[29] D. D., A. R. Malgwi, and H. S. Sambo, “Growth inhibitory effect of Senna siamea leaf extracts on selected microorganisms,” American Journal of Medicine and Medical Sciences, vol. 3, no. 5, pp. 103–107, 2013, doi: 10.5923/j.ajmms.20130305.02.

[30] S. I. Fadhila, E. K. Hayati, M. Rafi, and A. Sabarudin, “Effect of ethanol-water concentration as extraction solvent on antioxidant activity of Acalypha indica,” vol. 10, no. 2, pp. 133–142, 2023, doi: 10.15575/ak.v10i2.25573.

[31] F. Teshale, K. Narendiran, S. M. Beyan, and N. R. Srinivasan, “Extraction of essential oil from rosemary leaves: Optimization by response surface methodology and mathematical modeling,” Applied Food Research, vol. 2, no. 2, Art. no. 100133, Dec. 2022, doi: 10.1016/j.afres.2022.100133.

[32] J. R. Srinivasan and Y. Kawamura, “Rosemary extract: 82nd JECFA chemical and technical assessment,” JECFA Chemical and Technical Assessments, 2016.

[33] A. J. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, 3rd ed. Dordrecht, The Netherlands: Springer, 1998. [Online]. Available: https://link.springer.com/book/9780412572609.

[34] C. Bergeron, S. Gafner, E. Clausen, and D. J. Carrier, “Comparison of the chemical composition of extracts from Scutellaria lateriflora using accelerated solvent extraction and supercritical fluid extraction versus standard hot water or 70% ethanol extraction,” Journal of Agricultural and Food Chemistry, vol. 53, no. 8, pp. 3076–3080, Apr. 2005, doi: 10.1021/jf048408t.

[35] D. Nurhasanah, R. Ulvia, and F. Junita, “The effect of ethanol concentration variations on the total phenolic and flavonoid levels of Bauhinia purpurea L. leaf extract,” Journal of Biotechnology and Natural Science, vol. 4, no. 2, pp. 81–90, Dec. 2024, doi: 10.12928/JBNS.V4I2.12060.

[36] P. Arya, D. Vaidya, M. Kaushal, S. Devi, A. Gupta, and S. Chand, “Effects of different solvents on phytochemical constituents, in vitro antimicrobial activity, and volatile components of Boehmeria rugulosa Wedd. wood extract,” Scientific Reports, vol. 15, no. 1, Art. no. 29135, Aug. 2025, doi: 10.1038/s41598-025-14506-x.

[37] R. Kaundal, R. Kumar, and D. Kumar, “Optimized extraction technique and solvent to enhance the recovery of bioactive polyphenols from Viola canescens Wall. ex Roxb.: A case study using OFAT and chemical characterization using UPLC-PDA, UHPLC-Q-TOF-IMS, and GC–MS,” Microchemical Journal, vol. 212, Art. no. 113170, May 2025, doi: 10.1016/j.microc.2025.113170.

[38] N. Saewan and A. Jimtaisong, “Photoprotection of natural flavonoids,” Journal of Applied Pharmaceutical Science, vol. 3, no. 9, pp. 129–141, 2013, doi: 10.7324/JAPS.2013.3923.

[39] M. Taniguchi, C. A. LaRocca, J. D. Bernat, and J. S. Lindsey, “Digital database of absorption spectra of diverse flavonoids enables structural comparisons and quantitative evaluations,” Journal of Natural Products, vol. 86, no. 4, pp. 1087–1119, Apr. 2023, doi: 10.1021/acs.jnatprod.2c00720.

[40] P. K. Singh et al., “Rosemary bioactives as antioxidant agents: A bidirectional approach to improving human health and vegetable oil stability,” Food Chemistry Advances, vol. 7, Art. no. 100952, Jun. 2025, doi: 10.1016/j.focha.2025.100952.

[41] A. Khojasteh, M. H. Mirjalili, M. A. Alcalde, R. M. Cusido, R. Eibl, and J. Palazon, “Powerful plant antioxidants: A new biosustainable approach to the production of rosmarinic acid,” Antioxidants, vol. 9, no. 12, Art. no. 1273, Dec. 2020, doi: 10.3390/antiox9121273.

[42] J. Milutinov, N. Pavlović, D. Ćirin, M. Atanacković Krstonošić, and V. Krstonošić, “The potential of natural compounds in UV protection products,” Molecules, vol. 29, no. 22, Art. no. 5409, Nov. 2024, doi: 10.3390/molecules29225409.

[43] L. Li, L. Chong, T. Huang, Y. Ma, Y. Li, and H. Ding, “Natural products and extracts from plants as natural UV filters for sunscreens: A review,” Animal Models and Experimental Medicine, vol. 6, no. 3, pp. 183–195, Jun. 2022, doi: 10.1002/ame2.12295.