DETERMINION of DICLOFENAC SODIUM IN TABLETS FORMULATIONS by DEREVATIVE SPECTROPHOTOMETRY

Abstract

A new sensitive, simple, rapid, precise, accurate, economical and reproducible derivative spectrophotometric method has been developed. It requires no prior separation for the estimation of Diclofenac Sodium (Dic.Na) in tablets pharmaceutical form. First order spectrophotometric derivative method was adopted to eliminatespectral interference. The first order derivative absorption wavelength of (Dic.Na) was found to be 255 nm, dissolved in NaOH medium (1M). This wavelength was selected for the analysis of (Dic.Na) at 255 nm, obeyed Beer-Lambert's law in the concentrationsrange of 0.5 -32 μg/mL. Regression analysis showed a good correlation coefficient R2 = 0.9999. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.05 and 0.15 μg/mL respectively .The proposed method has been successfully applied in analyzing (Dic.Na) in some Syrian trademark drugs. All studied samples showed that the drug level was in conformity with the United State Pharmacopeia (USP) legislation