Studying the effect of growth hormone on creatinine levels and weight in rabbits: A pilot study

Abstract

Given the high rates of irresponsible misuse of growth hormone (GH) in sports for muscle mass gain and performance enhancement, it is crucial to investigate its potential side effects, particularly on renal function, which may be affected by such practices. This pilot study aimed to evaluate the effect of GH administration on body weight and plasma creatinine levels in adult male rabbits. Sixteen male rabbits, 7 months old and weighing an average of 2 kg, were divided into two equal groups (n=8): an untreated control group and a treatment group injected subcutaneously with GH at a dose of 16 IU daily for 10 days. After treatment, blood samples were drawn from the heart, plasma was separated, and creatinine levels were measured. The results showed a significant increase in creatinine in the GH group compared to the control group (4.93 ± 0.27 mg/dL vs. 2.05 ± 0.17 mg/dL, p<0.05). A clear and statistically significant increase in the weights of rabbits treated after injection compared to before injection was observed: 2.7±0.35 kg versus 1.91±0.22 kg (p<0.05). This reflects the strong anabolic effect of growth hormone, especially at high doses, associated with a significant weight gain and possible renal functional changes that go beyond just an increase in muscle mass, which necessitates strict monitoring of renal indicators during its use.