تقييم النشاط المضاد للالتهاب مخبرياً لنبات Capparis spinosa باستخدام طريقة تثبيط تمسخ البروتين

محمد فراس مناع; نور عشيش; ايمان فتوى; اسراء المصطفى; بتول بكار; حلا الشب

doi:10.5281/zenodo.20960807

Authors

Dr. Mohammd Firas Mannaa Al Wataniya Private University
Nour Ashesh Al Wataniya Private University
Eman Alfatwa Al Wataniya Private University
Esraa Almustafa Al Wataniya Private University
Batoul Bakkar Al Wataniya Private University
Hala Alshab Al Wataniya Private University

DOI:

https://doi.org/10.5281/zenodo.20960807

Keywords:

Methanol, Albumin denaturation, Anti-inflammatory Activity, Capparis Spinosa

Abstract

In traditional medicine, many natural plant-derived compounds have been used for centuries to inhibit inflammatory pathways, often causing fewer side effects than synthetic drugs. Capparis spinosa has been traditionally used to treat various ailments; however, scientific studies on its pharmacological efficacy are limited.

This study aims to assess the in vitro anti-inflammatory activity of C.spinosa using the albumin denaturation inhibition method. Bioactive compounds were extracted from (flowers, roots, fruits, leaves, and seeds) using methanol. Extracts at different concentrations were tested for their ability to inhibit egg albumin denaturation. Diclofenac sodium, a non-steroidal anti-inflammatory drug, served as the positive control. The anti-inflammatory effect was evaluated by calculating the percentage inhibition of protein denaturation.

The results showed a concentration-dependent increase in inhibition. Notably, the flower and root extracts exhibited significantly higher anti-inflammatory activity compared to other plant parts. Moreover, the flower extract demonstrated a markedly greater inhibition rate than the positive control.

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